Biobrick Restriction Enzyme
From 2007.igem.org
(Difference between revisions)
| Line 11: | Line 11: | ||
3'---CTTAA G---5' | 3'---CTTAA G---5' | ||
|- | |- | ||
| - | |[[XbaI|''Xba''I]] | + | |[[XbaI|''Xba''I]]||''[[Xanthomonas badrii]]'' |
| | | | ||
5'TCTAGA | 5'TCTAGA | ||
| Line 21: | Line 21: | ||
|colspan=4|* = blunt ends | |colspan=4|* = blunt ends | ||
|- | |- | ||
| - | |[[PstI|''Pst''I]] | + | |[[PstI|''Pst''I]]||''[[Providencia stuartii]]'' |
| | | | ||
5'CTGCAG | 5'CTGCAG | ||
| Line 28: | Line 28: | ||
5'---CTGCA G---3' | 5'---CTGCA G---3' | ||
3'---G ACGTC---5' | 3'---G ACGTC---5' | ||
| + | |- | ||
| + | |[[SpeI|''SpeI''I]]||''[[]]'' | ||
| + | | | ||
| + | 5' | ||
| + | 3' | ||
| + | | | ||
| + | 5'--- ---3' | ||
| + | 3'--- ---5' | ||
|- | |- | ||
|} | |} | ||
Revision as of 12:46, 20 September 2007
We have selected and for promoters and coding sequences insertion. Restriction enzymes are named based on the bacteria in which they are isolated in the following manner.
| Enzyme | Source | Recognition Sequence | Cut |
|---|---|---|---|
| EcoRI | Escherichia coli |
5'GAATTC 3'CTTAAG |
5'---G AATTC---3' 3'---CTTAA G---5' |
| XbaI | Xanthomonas badrii |
5'TCTAGA 3'AGATCT |
5'---T CTAGA---3' 3'---AGATC T---5' |
| * = blunt ends | |||
| PstI | Providencia stuartii |
5'CTGCAG 3'GACGTC |
5'---CTGCA G---3' 3'---G ACGTC---5' |
| SpeII | [[]] |
5' 3' |
5'--- ---3' 3'--- ---5' |
