Biobrick Restriction Enzyme
From 2007.igem.org
(Difference between revisions)
Line 31: | Line 31: | ||
|[[SpeI|''SpeI''I]]||''[[]]'' | |[[SpeI|''SpeI''I]]||''[[]]'' | ||
| | | | ||
- | 5' | + | 5'ACTAGT |
- | 3' | + | 3'TGATCA |
| | | | ||
- | 5'--- ---3' | + | 5'---A CTAGT---3' |
- | 3'--- ---5' | + | 3'---TGATC A---5' |
|- | |- | ||
|} | |} |
Revision as of 12:52, 20 September 2007
We have selected and for promoters and coding sequences insertion. Restriction enzymes are named based on the bacteria in which they are isolated in the following manner.
Enzyme | Source | Recognition Sequence | Cut |
---|---|---|---|
EcoRI | Escherichia coli |
5'GAATTC 3'CTTAAG |
5'---G AATTC---3' 3'---CTTAA G---5' |
XbaI | Xanthomonas badrii |
5'TCTAGA 3'AGATCT |
5'---T CTAGA---3' 3'---AGATC T---5' |
* = blunt ends | |||
PstI | Providencia stuartii |
5'CTGCAG 3'GACGTC |
5'---CTGCA G---3' 3'---G ACGTC---5' |
SpeII | [[]] |
5'ACTAGT 3'TGATCA |
5'---A CTAGT---3' 3'---TGATC A---5' |