Biobrick Restriction Enzyme
From 2007.igem.org
(Difference between revisions)
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3'---G ACGTC---5' | 3'---G ACGTC---5' | ||
|- | |- | ||
| - | |[[SpeI|''SpeI''I]]||'' | + | |[[SpeI|''SpeI''I]]||'''' |
| | | | ||
5'ACTAGT | 5'ACTAGT | ||
Revision as of 12:52, 20 September 2007
We have selected and for promoters and coding sequences insertion. Restriction enzymes are named based on the bacteria in which they are isolated in the following manner.
| Enzyme | Source | Recognition Sequence | Cut |
|---|---|---|---|
| EcoRI | Escherichia coli |
5'GAATTC 3'CTTAAG |
5'---G AATTC---3' 3'---CTTAA G---5' |
| XbaI | Xanthomonas badrii |
5'TCTAGA 3'AGATCT |
5'---T CTAGA---3' 3'---AGATC T---5' |
| * = blunt ends | |||
| PstI | Providencia stuartii |
5'CTGCAG 3'GACGTC |
5'---CTGCA G---3' 3'---G ACGTC---5' |
| SpeII | ' |
5'ACTAGT 3'TGATCA |
5'---A CTAGT---3' 3'---TGATC A---5' |
