Biobrick Restriction Enzyme
From 2007.igem.org
(Difference between revisions)
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| - | We have selected | + | We have selected XbaI and SpeI for promoters and coding sequences insertion. Restriction enzymes are named based on the bacteria in which they are isolated in the following manner. |
{| border="1" | {| border="1" | ||
!Enzyme!!Source!!Recognition Sequence!!Cut | !Enzyme!!Source!!Recognition Sequence!!Cut | ||
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3'---CTTAA G---5' | 3'---CTTAA G---5' | ||
|- | |- | ||
| - | |[[XbaI|''Xba''I]] | + | |[[XbaI|''Xba''I]]||''[[Xanthomonas badrii]]'' |
| | | | ||
5'TCTAGA | 5'TCTAGA | ||
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|colspan=4|* = blunt ends | |colspan=4|* = blunt ends | ||
|- | |- | ||
| - | |[[PstI|''Pst''I]] | + | |[[PstI|''Pst''I]]||''[[Providencia stuartii]]'' |
| | | | ||
5'CTGCAG | 5'CTGCAG | ||
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5'---CTGCA G---3' | 5'---CTGCA G---3' | ||
3'---G ACGTC---5' | 3'---G ACGTC---5' | ||
| + | |- | ||
| + | |[[SpeI|''SpeI''I]]||'''' | ||
| + | | | ||
| + | 5'ACTAGT | ||
| + | 3'TGATCA | ||
| + | | | ||
| + | 5'---A CTAGT---3' | ||
| + | 3'---TGATC A---5' | ||
|- | |- | ||
|} | |} | ||
Latest revision as of 12:57, 20 September 2007
We have selected XbaI and SpeI for promoters and coding sequences insertion. Restriction enzymes are named based on the bacteria in which they are isolated in the following manner.
| Enzyme | Source | Recognition Sequence | Cut |
|---|---|---|---|
| EcoRI | Escherichia coli |
5'GAATTC 3'CTTAAG |
5'---G AATTC---3' 3'---CTTAA G---5' |
| XbaI | Xanthomonas badrii |
5'TCTAGA 3'AGATCT |
5'---T CTAGA---3' 3'---AGATC T---5' |
| * = blunt ends | |||
| PstI | Providencia stuartii |
5'CTGCAG 3'GACGTC |
5'---CTGCA G---3' 3'---G ACGTC---5' |
| SpeII | ' |
5'ACTAGT 3'TGATCA |
5'---A CTAGT---3' 3'---TGATC A---5' |
