Biobrick Restriction Enzyme

From 2007.igem.org

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We have selected and for promoters and coding sequences insertion. Restriction enzymes are named based on the bacteria in which they are isolated in the following manner.
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We have selected XbaI and SpeI for promoters and coding sequences insertion. Restriction enzymes are named based on the bacteria in which they are isolated in the following manner.
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!Enzyme!!Source!!Recognition Sequence!!Cut
!Enzyme!!Source!!Recognition Sequence!!Cut

Latest revision as of 12:57, 20 September 2007

We have selected XbaI and SpeI for promoters and coding sequences insertion. Restriction enzymes are named based on the bacteria in which they are isolated in the following manner.

EnzymeSourceRecognition SequenceCut
EcoRIEscherichia coli
5'GAATTC
3'CTTAAG
5'---G     AATTC---3'
3'---CTTAA     G---5'
XbaIXanthomonas badrii
5'TCTAGA
3'AGATCT
5'---T  CTAGA---3'
3'---AGATC  T---5'
* = blunt ends
PstIProvidencia stuartii
5'CTGCAG
3'GACGTC
5'---CTGCA  G---3'
3'---G  ACGTC---5'
SpeII'
5'ACTAGT
3'TGATCA
5'---A  CTAGT---3'
3'---TGATC  A---5'