Biobrick Restriction Enzyme
From 2007.igem.org
(Difference between revisions)
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We have selected and for promoters and coding sequences insertion. Restriction enzymes are named based on the bacteria in which they are isolated in the following manner. | We have selected and for promoters and coding sequences insertion. Restriction enzymes are named based on the bacteria in which they are isolated in the following manner. | ||
| + | {| border="1" | ||
| + | !Enzyme!!Source!!Recognition Sequence!!Cut | ||
| + | |- | ||
| + | |[[EcoRI|''Eco''RI]]||''[[Escherichia coli]]'' | ||
| + | | | ||
| + | 5'GAATTC | ||
| + | 3'CTTAAG | ||
| + | | | ||
| + | 5'---G AATTC---3' | ||
| + | 3'---CTTAA G---5' | ||
| + | |- | ||
| + | |[[XbaI|''Xba''I]]<ref name=h/>||''[[Xanthomonas badrii]]'' | ||
| + | | | ||
| + | 5'TCTAGA | ||
| + | 3'AGATCT | ||
| + | | | ||
| + | 5'---T CTAGA---3' | ||
| + | 3'---AGATC T---5' | ||
| + | |- | ||
| + | |colspan=4|* = blunt ends | ||
| + | |- | ||
| + | |[[PstI|''Pst''I]]<ref name=h/>||''[[Providencia stuartii]]'' | ||
| + | | | ||
| + | 5'CTGCAG | ||
| + | 3'GACGTC | ||
| + | | | ||
| + | 5'---CTGCA G---3' | ||
| + | 3'---G ACGTC---5' | ||
| + | |- | ||
| + | |} | ||
Revision as of 11:34, 20 September 2007
We have selected and for promoters and coding sequences insertion. Restriction enzymes are named based on the bacteria in which they are isolated in the following manner.
| Enzyme | Source | Recognition Sequence | Cut |
|---|---|---|---|
| EcoRI | Escherichia coli |
5'GAATTC 3'CTTAAG |
5'---G AATTC---3' 3'---CTTAA G---5' |
| XbaI<ref name=h/> | Xanthomonas badrii |
5'TCTAGA 3'AGATCT |
5'---T CTAGA---3' 3'---AGATC T---5' |
| * = blunt ends | |||
| PstI<ref name=h/> | Providencia stuartii |
5'CTGCAG 3'GACGTC |
5'---CTGCA G---3' 3'---G ACGTC---5' |
