Biobrick Restriction Enzyme

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Revision as of 12:52, 20 September 2007 by Robertina (Talk | contribs)

We have selected and for promoters and coding sequences insertion. Restriction enzymes are named based on the bacteria in which they are isolated in the following manner.

EnzymeSourceRecognition SequenceCut
EcoRIEscherichia coli
5'GAATTC
3'CTTAAG
5'---G     AATTC---3'
3'---CTTAA     G---5'
XbaIXanthomonas badrii
5'TCTAGA
3'AGATCT
5'---T  CTAGA---3'
3'---AGATC  T---5'
* = blunt ends
PstIProvidencia stuartii
5'CTGCAG
3'GACGTC
5'---CTGCA  G---3'
3'---G  ACGTC---5'
SpeII[[]]
5'ACTAGT
3'TGATCA
5'---A  CTAGT---3'
3'---TGATC  A---5'