Biobrick Restriction Enzyme

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We have selected XbaI and SpeI for promoters and coding sequences insertion. Restriction enzymes are named based on the bacteria in which they are isolated in the following manner.

EnzymeSourceRecognition SequenceCut
EcoRIEscherichia coli 
5'GAATTC
3'CTTAAG

5'---G     AATTC---3'
3'---CTTAA     G---5'
XbaIXanthomonas badrii 
5'TCTAGA
3'AGATCT

5'---T  CTAGA---3'
3'---AGATC  T---5'
* = blunt ends
PstIProvidencia stuartii 
5'CTGCAG
3'GACGTC

5'---CTGCA  G---3'
3'---G  ACGTC---5'
SpeII' 
5'ACTAGT
3'TGATCA

5'---A  CTAGT---3'
3'---TGATC  A---5'
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