Biobrick Vector choice

From 2007.igem.org

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[[Image:Psb1A3map.jpg]]  [[Image:Gfp-report2.jpg]]
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We have selected from BioBrick plate the pSB1A3 plasmid to clone 1Xore and 2Xore promoters and pho4 coding sequence.
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This is a high copy number plasmid (copy number of 100-300 per cell) carrying ampicillin resistance.
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                  [[Image:Psb1A3map.jpg]]   
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Instead we have selected J61002 and J61003 plasmids to clone respective Pho80 and Pho8 coding sequence.
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We decided to insert a promoter element between EcoRI and XbaI and Pho80 or Ph08 Cds with XbaI and SpeI.
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                    [[Image:Gfp-report2.jpg]]  [[Image:Rfp-reporter.jpg]]

Latest revision as of 11:35, 23 October 2007

We have selected from BioBrick plate the pSB1A3 plasmid to clone 1Xore and 2Xore promoters and pho4 coding sequence. This is a high copy number plasmid (copy number of 100-300 per cell) carrying ampicillin resistance.

                 Psb1A3map.jpg   

Instead we have selected J61002 and J61003 plasmids to clone respective Pho80 and Pho8 coding sequence. We decided to insert a promoter element between EcoRI and XbaI and Pho80 or Ph08 Cds with XbaI and SpeI.

                   Gfp-report2.jpg  Rfp-reporter.jpg