Biobrick Vector choice
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| - | + | We have selected from BioBrick plate the pSB1A3 plasmid to clone 1Xore and 2Xore promoters and pho4 coding sequence. | |
| + | This is a high copy number plasmid (copy number of 100-300 per cell) carrying ampicillin resistance. | ||
| + | [[Image:Psb1A3map.jpg]] | ||
| + | Instead we have selected J61002 and J61003 plasmids to clone respective Pho80 and Pho8 coding sequence. | ||
| + | We decided to insert a promoter element between EcoRI and XbaI and Pho80 or Ph08 Cds with XbaI and SpeI. | ||
| - | + | [[Image:Gfp-report2.jpg]] [[Image:Rfp-reporter.jpg]] | |
| - | + | ||
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Latest revision as of 11:35, 23 October 2007
We have selected from BioBrick plate the pSB1A3 plasmid to clone 1Xore and 2Xore promoters and pho4 coding sequence. This is a high copy number plasmid (copy number of 100-300 per cell) carrying ampicillin resistance.
Instead we have selected J61002 and J61003 plasmids to clone respective Pho80 and Pho8 coding sequence. We decided to insert a promoter element between EcoRI and XbaI and Pho80 or Ph08 Cds with XbaI and SpeI.
