Biobrick Vector choice

From 2007.igem.org

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We have selected from BioBrick pSB1A3 plasmid to clone 1Xore and 2Xore promoters and pho4 coding sequence.
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We have selected from BioBrick plate the pSB1A3 plasmid to clone 1Xore and 2Xore promoters and pho4 coding sequence.
This is a high copy number plasmid (copy number of 100-300 per cell) carrying ampicillin resistance.
This is a high copy number plasmid (copy number of 100-300 per cell) carrying ampicillin resistance.
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Instead we have selected J61002 and J61003 plasmids to clone respective Pho80 and Pho8 coding sequence.
Instead we have selected J61002 and J61003 plasmids to clone respective Pho80 and Pho8 coding sequence.
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We have think to insert a promoter element between EcoRI and XbaI and Pho80 or Ph08 Cds with XbaI and SpeI.
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We decided to insert a promoter element between EcoRI and XbaI and Pho80 or Ph08 Cds with XbaI and SpeI.
                     [[Image:Gfp-report2.jpg]]  [[Image:Rfp-reporter.jpg]]
                     [[Image:Gfp-report2.jpg]]  [[Image:Rfp-reporter.jpg]]

Latest revision as of 11:35, 23 October 2007

We have selected from BioBrick plate the pSB1A3 plasmid to clone 1Xore and 2Xore promoters and pho4 coding sequence. This is a high copy number plasmid (copy number of 100-300 per cell) carrying ampicillin resistance.

                 Psb1A3map.jpg   

Instead we have selected J61002 and J61003 plasmids to clone respective Pho80 and Pho8 coding sequence. We decided to insert a promoter element between EcoRI and XbaI and Pho80 or Ph08 Cds with XbaI and SpeI.

                   Gfp-report2.jpg  Rfp-reporter.jpg