Bologna University/Digestion reaction
From 2007.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| + | :: (final volume 30μl) | ||
| + | |||
::1. Mix : | ::1. Mix : | ||
| - | ::::: -1 μg of plasmid | + | ::::: - 1 μg of plasmid |
| - | ::::: -3 μl of Buffer 10X | + | ::::: - 3 μl of Buffer 10X |
| - | ::::: -0.3 μl BSA 100X | + | ::::: - 0.3 μl BSA 100X |
| - | ::::: -3 units of restriction enzyme | + | ::::: - 3 units of restriction enzyme |
| - | ::::: -water up to 30 μl | + | ::::: - water up to 30 μl |
::2. Incubate at 37 °C for 1 hr. | ::2. Incubate at 37 °C for 1 hr. | ||
::3. Incubate at the inactivation temperature of the enzyme for 20 minutes. | ::3. Incubate at the inactivation temperature of the enzyme for 20 minutes. | ||
Revision as of 11:09, 3 August 2007
- (final volume 30μl)
- 1. Mix :
- - 1 μg of plasmid
- - 3 μl of Buffer 10X
- - 0.3 μl BSA 100X
- - 3 units of restriction enzyme
- - water up to 30 μl
- 2. Incubate at 37 °C for 1 hr.
- 3. Incubate at the inactivation temperature of the enzyme for 20 minutes.
- 1. Mix :
