Bologna University/Digestion reaction

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< Bologna University(Difference between revisions)
 
Line 2: Line 2:
::1. Mix :
::1. Mix :
-
::::: - 1 μg of plasmid
+
::::: - 1μg plasmid
-
::::: - 3 μl of Buffer 10X [ ]
+
::::: - 3μl Buffer 10x [ ]
-
::::: - 0.3 μl BSA 100X [ ]
+
::::: - 0.3μl BSA 100x [ ]
::::: - 3 units of restriction enzyme
::::: - 3 units of restriction enzyme
-
::::: - water up to 30 μl
+
::::: - water up to 30μl
-
::2. Incubate at 37 °C for 1 hr.
+
::2. Incubate at 37 °C for 1hr.
-
::3. Incubate at the inactivation temperature of the enzyme for 20 minutes.
+
::3. Incubate at the inactivation temperature of the enzyme for 20min.
[[Bologna University/Protocols | Back]]
[[Bologna University/Protocols | Back]]

Latest revision as of 09:43, 25 October 2007

(final volume 30μl)
1. Mix :
- 1μg plasmid
- 3μl Buffer 10x [ ]
- 0.3μl BSA 100x [ ]
- 3 units of restriction enzyme
- water up to 30μl
2. Incubate at 37 °C for 1hr.
3. Incubate at the inactivation temperature of the enzyme for 20min.


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