# Bologna University/Fluorescence reliance on Promoter and Medium

### From 2007.igem.org

(Difference between revisions)

Guido Costa (Talk | contribs) |
Guido Costa (Talk | contribs) |
||

Line 2: | Line 2: | ||

We test bacteria with two different plasmids, [http://partsregistry.org/Part:BBa_I763004 I763004] and [http://partsregistry.org/Part:BBa_I763035 I763035], each of them into two different mediums, LB and M9, to evaluate the dependence of the output fluorescence intensity on both the promoter and the medium. The experimental setup conditions are the same for each test, and the OD value is mantained at 1.2. We take pictures from two spots on each of the five slides we prepare; then, we calculate the mean value for each slide and finally the mean and the standard devioation on these values. Here are the results: | We test bacteria with two different plasmids, [http://partsregistry.org/Part:BBa_I763004 I763004] and [http://partsregistry.org/Part:BBa_I763035 I763035], each of them into two different mediums, LB and M9, to evaluate the dependence of the output fluorescence intensity on both the promoter and the medium. The experimental setup conditions are the same for each test, and the OD value is mantained at 1.2. We take pictures from two spots on each of the five slides we prepare; then, we calculate the mean value for each slide and finally the mean and the standard devioation on these values. Here are the results: | ||

+ | |||

{| border="1" cellpadding="5" cellspacing="0" align="center" | {| border="1" cellpadding="5" cellspacing="0" align="center" |

## Revision as of 14:42, 24 October 2007

**Fluorescence reliance on Promoter and Medium**

We test bacteria with two different plasmids, I763004 and I763035, each of them into two different mediums, LB and M9, to evaluate the dependence of the output fluorescence intensity on both the promoter and the medium. The experimental setup conditions are the same for each test, and the OD value is mantained at 1.2. We take pictures from two spots on each of the five slides we prepare; then, we calculate the mean value for each slide and finally the mean and the standard devioation on these values. Here are the results:

LB | M9 | |
---|---|---|

I763004 | 102.9 ± 13.4 | 123.2 ± 13.1 |

I763035 | 106.6 ± 6.0 | 138.1 ± 7.7 |