Bologna University/Ligation reaction

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(Difference between revisions)
Line 4: Line 4:
::::: - 8 μl of insert DNA
::::: - 8 μl of insert DNA
::::: - 4 μl of vector plasmid
::::: - 4 μl of vector plasmid
-
::::: - 4 μl of Buffer 5X
+
::::: - 4 μl of Buffer 5X [ ]
::::: - 1 μl of T4 Ligase  
::::: - 1 μl of T4 Ligase  
-
::::: - 3 μl of mQ water  
+
::::: - 3 μl of ultrapure water  
::2. Incubate at 4 °C overnigth or at 15 °C for 3 hr.
::2. Incubate at 4 °C overnigth or at 15 °C for 3 hr.

Revision as of 09:31, 25 October 2007

(final volume 20 μl)
1. Mix :
- 8 μl of insert DNA
- 4 μl of vector plasmid
- 4 μl of Buffer 5X [ ]
- 1 μl of T4 Ligase
- 3 μl of ultrapure water
2. Incubate at 4 °C overnigth or at 15 °C for 3 hr.



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