From 2007.igem.org

Revision as of 11:10, 3 August 2007 (view source) Silvia.tamarri2 (Talk | contribs) ← Older edit		Latest revision as of 09:41, 25 October 2007 (view source) E-man (Talk | contribs)
(3 intermediate revisions not shown)
Line 1:		Line 1:
-	:: Not yet setted	+	:: (final volume 20 μl)
		+
		+	::1. Mix :
		+	::::: - 8μl of insert DNA
		+	::::: - 4μl of vector plasmid
		+	::::: - 4μl of Buffer 5x [ ]
		+	::::: - 1μl of T4 Ligase
		+	::::: - 3μl of ultrapure water
		+	::2. Incubate at 4 °C overnigth or at 15 °C for 3hr.
		+
		+
	[[Bologna University/Protocols | Back]]		[[Bologna University/Protocols | Back]]

---

Latest revision as of 09:41, 25 October 2007

(final volume 20 μl)

1. Mix :

- 8μl of insert DNA

- 4μl of vector plasmid

- 4μl of Buffer 5x [ ]

- 1μl of T4 Ligase

- 3μl of ultrapure water

2. Incubate at 4 °C overnigth or at 15 °C for 3hr.

Back