Bologna University/Ligation reaction
From 2007.igem.org
(Difference between revisions)
Line 4: | Line 4: | ||
::::: - 8 μl of insert DNA | ::::: - 8 μl of insert DNA | ||
::::: - 4 μl of vector plasmid | ::::: - 4 μl of vector plasmid | ||
- | ::::: - 4 μl of Buffer | + | ::::: - 4 μl of Buffer 5x [ ] |
::::: - 1 μl of T4 Ligase | ::::: - 1 μl of T4 Ligase | ||
::::: - 3 μl of ultrapure water | ::::: - 3 μl of ultrapure water | ||
- | ::2. Incubate at 4 °C overnigth or at 15 °C for | + | ::2. Incubate at 4 °C overnigth or at 15 °C for 3hr. |
Revision as of 09:41, 25 October 2007
- (final volume 20 μl)
- 1. Mix :
- - 8 μl of insert DNA
- - 4 μl of vector plasmid
- - 4 μl of Buffer 5x [ ]
- - 1 μl of T4 Ligase
- - 3 μl of ultrapure water
- 2. Incubate at 4 °C overnigth or at 15 °C for 3hr.
- 1. Mix :