Bologna University/Plates preparation

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< Bologna University(Difference between revisions)
 
Line 1: Line 1:
::1. Autoclave LB medium with 2% agar.
::1. Autoclave LB medium with 2% agar.
::2. Cool at 50 °C to prevent agar polymerization.  
::2. Cool at 50 °C to prevent agar polymerization.  
-
::3. Before preparing plates add antibiotic (Ampicillin 1000X [ ] or Kanamicin 200X [ ]).  
+
::3. Before preparing plates add antibiotic (Ampicillin 1000x [ ] or Kanamicin 200x [ ]).  
-
::4. Put about 20 ml of medium per plate.
+
::4. Put about 20ml of medium per plate.
::5. Leave it solidify and store at 4°C.
::5. Leave it solidify and store at 4°C.
[[Bologna University/Protocols | Back]]
[[Bologna University/Protocols | Back]]

Latest revision as of 09:45, 25 October 2007

1. Autoclave LB medium with 2% agar.
2. Cool at 50 °C to prevent agar polymerization.
3. Before preparing plates add antibiotic (Ampicillin 1000x [ ] or Kanamicin 200x [ ]).
4. Put about 20ml of medium per plate.
5. Leave it solidify and store at 4°C.


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