Bologna University/Procedure
From 2007.igem.org
(Difference between revisions)
| Line 7: | Line 7: | ||
::7. Miniprep (QIAprep Spin Miniprep kit - Ca No 27104 Qiagen) | ::7. Miniprep (QIAprep Spin Miniprep kit - Ca No 27104 Qiagen) | ||
::8. Stock preparation in 40% glycerol and digestion for control | ::8. Stock preparation in 40% glycerol and digestion for control | ||
| + | |||
| + | |||
[[Bologna | Back]] | [[Bologna | Back]] | ||
Revision as of 09:43, 3 August 2007
- 1. Biobricks amplification
- 2. Digestion reaction
- 3. Run on agarose 1% gel
- 4. DNA extraction from gel (QIAquick Gel Extraction kit – Ca No 28704)
- 5. Ligation reaction
- 6. Transformation
- 7. Miniprep (QIAprep Spin Miniprep kit - Ca No 27104 Qiagen)
- 8. Stock preparation in 40% glycerol and digestion for control
