From 2007.igem.org

1. Biobricks amplification

2. Digestion reaction

3. Run on agarose 1% gel

4. DNA extraction from gel (QIAquick Gel Extraction kit – Ca No 28704)

5. Ligation reaction

6. Transformation

7. Miniprep (QIAprep Spin Miniprep kit - Ca No 27104 Qiagen)

8. Stock preparation in 40% glycerol and digestion for control

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