Boston University
From 2007.igem.org
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## [[Design primers for amplification]] | ## [[Design primers for amplification]] | ||
## Perform error-prone PCR | ## Perform error-prone PCR | ||
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# Transform mutated genes into E. coli | # Transform mutated genes into E. coli | ||
## Choose plasmids | ## Choose plasmids | ||
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## Ligation | ## Ligation | ||
## Transformation into E. coli | ## Transformation into E. coli | ||
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# Conjugate E. coli with Shewanella | # Conjugate E. coli with Shewanella | ||
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# Screen/select for increased current production due to mutations | # Screen/select for increased current production due to mutations | ||
Revision as of 18:34, 29 May 2007
About Us
Welcome to the wiki for Boston University's iGEM 2007 team!
Our team consists of the following members:
Undergraduates: David Shi, Rahul Ahuja, Christian Ling, Danny Bellin
Faculty Advisor: Dr. Timothy Gardner
Graduate Student Advisors: Frank Juhn, Stephen Schneider
We work at the the Gardner Laboratory.
We are grateful to our advisors for taking us on and agreeing to help us out!
Our Project Plan
Our project is aimed at increasing current production from Shewanella Oneidensis by directed evolution of global transcription factors
Our plan so far:
- Mutate global transcription factors
- Design primers for amplification
- Perform error-prone PCR
- Transform mutated genes into E. coli
- Choose plasmids
- Restriction enzyme digestion
- Ligation
- Transformation into E. coli
- Conjugate E. coli with Shewanella
- Screen/select for increased current production due to mutations
Week's Goals
Wednesday 5/30
- Get all protocols
- Identify materials/prepare order
- Design Primers
- Learn about budget/POs
Thursday 5/31
- Do primer order
- Start conjugation practice
- Confirm restriction enzymes, ligases
- Order confirmed/needed materials
- Team Revew Meeting
Friday 6/1
- Evaluate/continue conjugation, practice electroporation for E. coli
- Meeting with Tim: Budgets/protocols, Pfizer/fundraising, iGEM registration, beads
