Boston University/Bacterial Conjugation Results

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[[Boston University/Filter Conjugation Protocol | Filter Conjugation Protocol]]
[[Boston University/Filter Conjugation Protocol | Filter Conjugation Protocol]]
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FIlter conjugation to transfer the plasmid () from E.coli SM10 to Shewanella AS-92
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Filter conjugation to transfer the plasmid pMS291(lacZ) from E.coli SM10 to S. oneidensis AS-92.
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The plasmid pMS291 contained a kanamycin resistance gene.  S. oneidensis has a native gentamicin resistance.
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The plasmid pMS291 is first transformed into E.coli SM10 and then the conjugation protocol is run to encourage conjugal transfer of the plasmid to S. oneidensis AS-92.  A double-antibiotic selection method is used to select for successful S. onedensis transformants which have a resistance for both kanamycin and gentamicin.
[[Image:BU_conjugation1.jpg]]
[[Image:BU_conjugation1.jpg]]

Revision as of 16:24, 16 July 2007

Filter Conjugation Protocol

Filter conjugation to transfer the plasmid pMS291(lacZ) from E.coli SM10 to S. oneidensis AS-92.

The plasmid pMS291 contained a kanamycin resistance gene. S. oneidensis has a native gentamicin resistance.

The plasmid pMS291 is first transformed into E.coli SM10 and then the conjugation protocol is run to encourage conjugal transfer of the plasmid to S. oneidensis AS-92. A double-antibiotic selection method is used to select for successful S. onedensis transformants which have a resistance for both kanamycin and gentamicin.

BU conjugation1.jpg

BU conjugation2.jpg

BU conjugation3.jpg

BU conjugation4.jpg

BU conjugation5.jpg

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