Boston University/Bacterial Conjugation Results
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[[Boston University/Filter Conjugation Protocol | Filter Conjugation Protocol]] | [[Boston University/Filter Conjugation Protocol | Filter Conjugation Protocol]] | ||
| - | Filter conjugation to transfer the plasmid pMS291(lacZ) from E.coli SM10 to S. oneidensis AS-92. | + | :Filter conjugation to transfer the plasmid pMS291(lacZ) from E.coli SM10 to S. oneidensis AS-92. |
| - | The plasmid pMS291 contained a kanamycin resistance gene. S. oneidensis has a native gentamicin resistance. | + | :The plasmid pMS291 contained a kanamycin resistance gene. S. oneidensis has a native gentamicin resistance. |
| - | The plasmid pMS291 is first transformed into E.coli SM10 and then the conjugation protocol is run to encourage conjugal transfer of the plasmid to S. oneidensis AS-92. A double-antibiotic selection method is used to select for successful S. onedensis transformants which have a resistance for both kanamycin and gentamicin. | + | :The plasmid pMS291 is first transformed into E.coli SM10 and then the conjugation protocol is run to encourage conjugal transfer of the plasmid to S. oneidensis AS-92. A double-antibiotic selection method is used to select for successful S. onedensis transformants which have a resistance for both kanamycin and gentamicin. |
[[Image:BU_conjugation1.jpg]] | [[Image:BU_conjugation1.jpg]] | ||
Revision as of 16:24, 16 July 2007
- Filter conjugation to transfer the plasmid pMS291(lacZ) from E.coli SM10 to S. oneidensis AS-92.
- The plasmid pMS291 contained a kanamycin resistance gene. S. oneidensis has a native gentamicin resistance.
- The plasmid pMS291 is first transformed into E.coli SM10 and then the conjugation protocol is run to encourage conjugal transfer of the plasmid to S. oneidensis AS-92. A double-antibiotic selection method is used to select for successful S. onedensis transformants which have a resistance for both kanamycin and gentamicin.
