Boston University/Plasmid Customization

From 2007.igem.org

(Difference between revisions)
(Excising the sacB gene from plasmid pJQ200)
(Verifying the presence of the lacZ promoter in the plasmid pJQ200)
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:[[Image:BU_labeled_pjq200_sacb_digestion_7-17.jpg]]
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===Verifying the presence of the lacZ promoter in the plasmid pJQ200===
 
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{| style="width:700pt;" border="1"
 
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:'''Double digestion of pJQ200 with EcoRI and HindIII'''
 
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::::TEXT
 
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[[Image:BU_labeled_lacz_PCR_cropped_7-17.jpg]]
 
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[[Image:BU_labeled_lacz_PCR_7-21-2007.jpg]]
 
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[[Image:BU_labeled_lacz_PCR_7-19-2007.jpg]]
 
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Revision as of 18:18, 23 July 2007

Excising the sacB gene from plasmid pJQ200

PCR amplification of lacZ





TEXT






BU labeled digested pjq200 7-19-2007.jpg
BU labeled pjq200 sacb digestion 7-17.jpg