Boston University/Plasmid Customization

From 2007.igem.org

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(→Excising the sacB gene from plasmid pJQ200)
(→'''Verifying the presence of the lacZ promoter in the plasmid pJQ200''')
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== '''Verifying the presence of the lacZ promoter in the plasmid pJQ200''' ==
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==Verifying the presence of the lacZ promoter in the plasmid pJQ200==

Revision as of 18:20, 23 July 2007

Excising the sacB gene from plasmid pJQ200

PCR amplification of lacZ





TEXT






BU labeled digested pjq200 7-19-2007.jpg
BU labeled pjq200 sacb digestion 7-17.jpg




Verifying the presence of the lacZ promoter in the plasmid pJQ200

Double digestion of pJQ200 with EcoRI and HindIII





TEXT






BU labeled lacz PCR cropped 7-17.jpg

BU labeled lacz PCR 7-21-2007.jpg

BU labeled lacz PCR 7-19-2007.jpg

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