Boston University/Plasmid Customization

From 2007.igem.org

(Difference between revisions)
(Verifying the presence of the lacZ promoter in the plasmid pJQ200)
Line 1: Line 1:
 +
 +
[[Boston University/Plasmid Selection and Design]]
 +
===Excising the sacB gene from plasmid pJQ200===
===Excising the sacB gene from plasmid pJQ200===

Revision as of 18:23, 23 July 2007

Boston University/Plasmid Selection and Design

Excising the sacB gene from plasmid pJQ200

PCR amplification of lacZ










BU labeled digested pjq200 7-19-2007.jpg
BU labeled pjq200 sacb digestion 7-17.jpg

Verifying the presence of the lacZ promoter in the plasmid pJQ200

Double digestion of pJQ200 with EcoRI and HindIII










BU labeled lacz PCR cropped 7-17.jpg

BU labeled lacz PCR 7-21-2007.jpg

BU labeled lacz PCR 7-19-2007.jpg