Boston University/Plasmid Customization

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(Overview)
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[[Boston University/Plasmid Selection and Design | Plasmid pJQ200]]
[[Boston University/Plasmid Selection and Design | Plasmid pJQ200]]
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===Excising the sacB gene from plasmid pJQ200===
===Excising the sacB gene from plasmid pJQ200===

Revision as of 18:24, 23 July 2007

Overview

Plasmid pJQ200

Excising the sacB gene from plasmid pJQ200

PCR amplification of lacZ










BU labeled digested pjq200 7-19-2007.jpg
BU labeled pjq200 sacb digestion 7-17.jpg

Verifying the presence of the lacZ promoter in the plasmid pJQ200

Double digestion of pJQ200 with EcoRI and HindIII










BU labeled lacz PCR cropped 7-17.jpg

BU labeled lacz PCR 7-21-2007.jpg

BU labeled lacz PCR 7-19-2007.jpg