Boston University/Plasmid Customization

From 2007.igem.org

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(Verifying the presence of the lacZ promoter in the plasmid pJQ200)
(Overview)
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===PCR amplification of the global transcription factors SO1415, SO4157, hlyU===
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[[Image:BU_labeled_GTF_PCR_7-25-2007.jpg]]
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Revision as of 14:49, 30 July 2007

Contents

Overview

Plasmid pJQ200

Excising the sacB gene from plasmid pJQ200

BU labeled digested pjq200 7-19-2007.jpg
BU labeled pjq200 sacb digestion 7-17.jpg






Verifying the presence of the lacZ promoter in the plasmid pJQ200

BU labeled lacz PCR cropped 7-17.jpg

BU labeled lacz PCR 7-21-2007.jpg

BU labeled lacz PCR 7-19-2007.jpg

BU labeled lacz PCR 7-24-2007.jpg

BU labeled digested pjq200 7-19-2007.jpg

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BU labeled pjq200 sacb digestion 7-17.jpg

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PCR amplification of the global transcription factors SO1415, SO4157, hlyU

BU labeled GTF PCR 7-25-2007.jpg