From 2007.igem.org

Revision as of 17:08, 9 July 2007 (view source) Linger (Talk | contribs) ← Older edit		Revision as of 16:51, 16 July 2007 (view source) Linger (Talk | contribs) (→Short-Term To-Do List) Newer edit →
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	== Short-Term To-Do List ==		== Short-Term To-Do List ==
	EDIT THE WIIIIKIIII		EDIT THE WIIIIKIIII
		+	Clone lacZ promoter into pJQ200
	== Protocols ==		== Protocols ==

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Revision as of 16:51, 16 July 2007

Contents 1 What We've Accomplished 2 Materials We Need 3 Short-Term To-Do List 4 Protocols 5 Question and Answer

What We've Accomplished

Plasmid Choice

Restriction Enzyme Choice

Primer Design

Making Shewanella Competent and Transforming Plasmid

Materials We Need

Error-Prone PCR: From CAB(?)

Ligases: Need to Buy

Short-Term To-Do List

EDIT THE WIIIIKIIII Clone lacZ promoter into pJQ200

Protocols

Calcium Chloride/Heat Shock Plasmid Transformations Protocol

Filter Cojugation Protocol

pTrcHis TOPO TA Expression Kit Cloning Protocol

Using NEBCutter for checking specific restriction enzymes against a sequence

Making an Electrophoresis Gel

Procedure for 50 mL E. Coli mixture in Zymo Broth or SOB

Question and Answer

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