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Revision as of 16:50, 16 July 2007
Introduction to Zymo Transformation and Testing
- E. Coli Transformants
- The image immediately to the right of this text shows the results from our transformation of E.coli with plasmids pBAD18s, pBAD30, and pjQ200 via Zymo transformation and electroporation.
- The contents of the plates are as follows(column indicates transformation type):
| Zymo(poured) | Zymo(streaked) | Electroporation
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| pBAD18s | pBAD18s | pBAD18s
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| pBAD30 | pBAD30 | pBAD30
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| pjQ200 | pjQ200 | pjQ200
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- The "poured" and "streaked" designations refer to how the transformed E. coli were put onto the plate. When transformed E. coli were streaked onto the plate, 5 microliters were used. When transformed E. coli were poured onto the plate, 100 microliters were used.
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- Shewy Transformants
- The image immediately to the right of this text shows the results from our transformation of S. oneidensis with plasmids pBAD18s, pBAD30, and pjQ200 via Zymo transformation and electroporation.
- The contents of the plates are as follows(column indicates transformation type):
| Zymo(streaked) | Zymo(poured) | Electroporation
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| pBAD18s | pBAD18s | pBAD18s
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| pBAD30 | pBAD30 | pBAD30
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| pjQ200 | pjQ200 | pjQ200
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- The "poured" and "streaked" designations refer to how the transformed Shewy were put onto the plate. When transformed Shewy were streaked onto the plate, 5 microliters were used. When transformed Shewy were poured onto the plate, 100 microliters were used.
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Overview of Zymo Transformation Results
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