Boston UniversityGel
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# Let it cool until its cool enough so you can touch it for an extended period of time. | # Let it cool until its cool enough so you can touch it for an extended period of time. | ||
# Add 4ul of Etbr and pour it on the gel-plate. | # Add 4ul of Etbr and pour it on the gel-plate. | ||
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| + | '''Loading''' | ||
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| + | Load 10ul of diluted ladder in a lane. | ||
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| + | The 1/40loading dye should have a final volume 1/5 in the overall solution . | ||
Latest revision as of 15:16, 5 July 2007
Making Electrophoresis Gel
- Take 60ml TAE 1X and add 0.6g agarose.
- Microwave for about 45 seconds.
- Let it cool until its cool enough so you can touch it for an extended period of time.
- Add 4ul of Etbr and pour it on the gel-plate.
Loading
Load 10ul of diluted ladder in a lane.
The 1/40loading dye should have a final volume 1/5 in the overall solution .
