From 2007.igem.org

Revision as of 15:30, 6 July 2007 (view source) Rahula (Talk | contribs) (→Question and Answer) ← Older edit		Latest revision as of 16:43, 6 July 2007 (view source) Rahula (Talk | contribs) (→Short-Term To-Do List)
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	== Short-Term To-Do List ==		== Short-Term To-Do List ==
-		+	EDIT THE WIIIIKIIII
-	David:	+
-	1)Look up metal-sensitive/redox-sensitive chelating dyes for Tim's grant	+
-	2)Look up how to control alginate bead size for Tim's grant	+
-	3)Begin primer design for pJQ200 (regulator, promoter, global transcription	+
-	regulators)	+
-	4)send stuff to Danny for wiki update	+
-	5)grow overnight of the shewie zymo for miniprep tomorrow	+
-		+
-	Rahul:	+
-	1)Work on compiling documents, protocols, results and send to Danny for Wiki	+
-	updates	+
-	2)Understand error-prone PCR and write a short summary/outline of important	+
-	details and key steps (and problem areas and controls)	+
-		+
-	Christian:	+
-	1)Decide on promoters, regulators to put into pJQ200	+
-	2)document this stuff so we can wiki it	+
-		+
-	Danny:	+
-	1)Wiki needs a format update /new look.  we'll email some suggestions for you	+
-	to	+
-	look at later today	+
-	2)receive info from us and update wiki	+
	== Protocols ==		== Protocols ==

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Latest revision as of 16:43, 6 July 2007

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Contents 1 What We've Accomplished 2 Materials We Need 3 Short-Term To-Do List 4 Protocols 5 Question and Answer

What We've Accomplished

Plasmid Choice

Restriction Enzyme Choice

Primer Design

Making Shewanella Competent and Transforming Plasmid

Materials We Need

Error-Prone PCR: From CAB(?)

Ligases: Need to Buy

Short-Term To-Do List

EDIT THE WIIIIKIIII

Protocols

Calcium Chloride/Heat Shock Plasmid Transformations Protocol

Filter Cojugation Protocol

pTrcHis TOPO TA Expression Kit Cloning Protocol

Using NEBCutter for checking specific restriction enzymes against a sequence

Making an Electrophoresis Gel

Procedure for 50 mL E. Coli mixture in Zymo Broth or SOB

Question and Answer

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