Calgary

From 2007.igem.org

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<span style="font-size:18px">Welcome to the  University of Calgary Wiki for the 2007 iGEM Competition</span>
<span style="font-size:18px">Welcome to the  University of Calgary Wiki for the 2007 iGEM Competition</span>
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<p>This site presents detailed information about both of our projects. We have two projects that we are entering in this compeition. The first <b>Eco. Lisa</b> is our biological entry, the second <b>Evo Gem</b> is our computational project. Full descriptions of each project can be found by using the links to your left. We are updating this page with new information for the public on an ongoing basis!</p>
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<p>This site presents detailed information about both of our projects. We have two projects that we are entering in this compeition. The first <b>Eco. Lisa</b> is our biological entry, the second <b>Evo Gem</b> is our computational project. The overal theme of our entry this year is designing highly controlled systems that allow for human-bacteria interaction. Full descriptions of each project can be found by using the links to your left. </p>
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<span style="font-size:18px">E. coLisa What It Is and Where We Are...</span>
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<span style="font-size:18px">E. coLisa!</span>
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<p>The primary UofC iGEM project for 2007 is to design and build a biomechanical printer; composed of a two dimensional plotter equipped with a red laser, software to translate computer images into instructions for the plotter, and E. coli cells engineered to respond to the laser light. Bacteria are spread in a solid lawn on the plate, or mixed in the media before pouring the plate. The response triggered by this biological circuit is to produce beta agarase, an enzyme which degrades the agar polymer that the cells rest on.</p>
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<img src="https://static.igem.org/mediawiki/2007/e/e3/GlowingBacteria.jpg" alt="laser shining on bacteria" />
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By varying the amount of light exposure each point on the plate receives, we can control the amount of agarase expression, and so the height of the agar gel, at each location. The result is bacterial lithography. In particular, the fine resolution achievable by laser control could allow resolution as high as 10 megapixels per inch, precise enough to target individual cells. </p>
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Our <em>E. coLisa</em> project is our teams main project this year. Our goal is to engineer <em>E. coli</em> so that when we shine a light on them they will relase a protein called agarase. The agarase will then dissolve the agar medium in which the bacteria rest. We want to use this sytem to dissolve images into agar plates to create very high resolution pictures.
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<p>At the moment we have our logic circut builit and placed in a biobrick plasmid. Agarase is still in the process of being isolated and our light sensing component is still understruction</p></td>
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<span style="font-size:18px">evoGEM What It Is and Where We Are...</span>
<span style="font-size:18px">evoGEM What It Is and Where We Are...</span>

Revision as of 04:24, 27 October 2007

E. coLisa

Project
Design: Wet Lab
Design: Printer
Design: Software
Testing
Constructing Our Project: The Wetlab
Protocols
Final Result of E. coLisa

evoGEM

Introduction to evoGEM
Project Design
Results
Welcome to the University of Calgary Wiki for the 2007 iGEM Competition

This site presents detailed information about both of our projects. We have two projects that we are entering in this compeition. The first Eco. Lisa is our biological entry, the second Evo Gem is our computational project. The overal theme of our entry this year is designing highly controlled systems that allow for human-bacteria interaction. Full descriptions of each project can be found by using the links to your left.

E. coLisa!
laser shining on bacteria

Our E. coLisa project is our teams main project this year. Our goal is to engineer E. coli so that when we shine a light on them they will relase a protein called agarase. The agarase will then dissolve the agar medium in which the bacteria rest. We want to use this sytem to dissolve images into agar plates to create very high resolution pictures.

evoGEM What It Is and Where We Are...

At this point our evoGEM project is ready to show. It will accept a variety of parametres and automatically begin to generate potential circuts