Chiba/Communication

From 2007.igem.org

(Difference between revisions)
(Our Aim)
Line 11: Line 11:
==Communication Unit==
==Communication Unit==
===Our Aim===
===Our Aim===
-
[[Image:Chiba_prj_com.png|frame|'''Fig1.''' Quorum sensing for activating receivers!]]
+
[[Image:Chiba_prj_com.png|frame|'''Fig13.''' Quorum sensing for activating receivers!]]
Our aim is to make bacteria stick around a bacteria core.  
Our aim is to make bacteria stick around a bacteria core.  
Make a sender, a bacteria core that sends a signal, and a receiver, a bacteria that receive a signal.
Make a sender, a bacteria core that sends a signal, and a receiver, a bacteria that receive a signal.

Revision as of 05:04, 27 October 2007

Chiba logo.png

Introduction | Project Design ( 1.Affinity Tag | 2.Communication Module | 3.Size Control ) | Making Marimos | Our Goal
Acknowledgements | Team Members | iGEM Chiba Website | メンバ連絡簿


Communication Unit

Our Aim

Fig13. Quorum sensing for activating receivers!

Our aim is to make bacteria stick around a bacteria core. Make a sender, a bacteria core that sends a signal, and a receiver, a bacteria that receive a signal. Our system works like this: Sender core, which is sticking each other in advance, sends a signal to receivers, which grow stickey tags. Then they stick together. We use Quorum sensing as a signal.

About Quorum Sensing

Fig2. Constructed circuit of Quorum sensing in our system.

Quorum sensing is a cell-to-cell signaling action of bacteria. They detect the cell density of the same species and coordinate the expression behavior of their cells. Species of Gram-Negative signaling transfer molecules (so-called autoinducer) is a series of acyl homoserine lactone (AHL). When the AHL concentration exceeds a threshold, that is, the cell population is over a certain number, the promoter plux transcription is activated.

Detailed gene circuit is discribed in Fig. 2.

  • more about quorum sensing

Cell-Cell-Signaling

About Quorum sensing

Experiments: New Parts!!

Recievers

New Parts We Made!!

From Biobrick

  • BBa_T9002
    • T9002 circuit.jpg

Future Work

  • inverter-aiiA
    • Rec inv aiia.jpg

Senders

New Parts We Made

  • MetK Sender ( could not deposit to registry )

MetK circuit.jpg

From Biobrick

  • Sender(BBa_S03623)

BBa s03623.jpg