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Revision as of 02:58, 2 September 2007 (view source) Masahiro (Talk | contribs) ← Older edit		Revision as of 03:09, 2 September 2007 (view source) Masahiro (Talk | contribs) Newer edit →
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	== FliC TEAM ==		== FliC TEAM ==
-	#LINKER LIGATION	+	*LINKER LIGATION
-	##inverse PCR	+	**pUC19-FliC(vector)の制作
		+	#Inverse PCR（五種類のF-Primer,R-Primer→15通りのPCR）
		+	##Check Size
		+	##Digestion(EcoRI,NcoI)
		+	##Zymo Clean(40μ溶出)→SAP（50μ系）→Zymo Clean（30μ溶出）
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	##Digestion Test		##Digestion Test
	##Sequence		##Sequence
-
-
	#Expression Test of a FliC-Histag		#Expression Test of a FliC-Histag
	##SDS-PAGE		##SDS-PAGE
	##Western-Blotting		##Western-Blotting
	##Magnetic beads		##Magnetic beads

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Revision as of 03:09, 2 September 2007

Lux TEAM

FliC TEAM

• LINKER LIGATION
  • pUC19-FliC(vector)の制作

1. Inverse PCR（五種類のF-Primer,R-Primer→15通りのPCR）
   1. Check Size
   2. Digestion(EcoRI,NcoI)
   3. Zymo Clean(40μ溶出)→SAP（50μ系）→Zymo Clean（30μ溶出）

1. lasmid check
   1. Digestion Test
   2. Sequence
2. Expression Test of a FliC-Histag
   1. SDS-PAGE
   2. Western-Blotting
   3. Magnetic beads