Composite part of hemA from R. capsulatus and RBS
From 2007.igem.org
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| - | Composite part of RBS+hemA from R.capsulatus | + | Composite part of RBS (pBca1101-I716051-kan cassette) + hemA from R.capsulatus |
Digest pBca9145-I716151 (Bg1II/XhoI, 2063+1221, S) | Digest pBca9145-I716151 (Bg1II/XhoI, 2063+1221, S) | ||
| - | Sub into | + | Sub into pBca1101-I716051 (BamH1/XhoI, 3119+850, L) |
Product is I716158 | Product is I716158 | ||
| - | + | </pre> | |
Latest revision as of 20:52, 18 June 2007
Composite part of RBS (pBca1101-I716051-kan cassette) + hemA from R.capsulatus Digest pBca9145-I716151 (Bg1II/XhoI, 2063+1221, S) Sub into pBca1101-I716051 (BamH1/XhoI, 3119+850, L) Product is I716158
