Composite part of hemA from R.capsulatus and RBS pBca1101-1106A

From 2007.igem.org

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Composite part of RBS (pBca1101-1106B) + hemA from R. capsulatus
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Composite part of RBS (pBca1101-1106A) + hemA from R. capsulatus
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Digest pBca1101-1106B         (BamH1/XhoI, 2132+850, L)
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Digest pBca1101-1106A         (BamH1/XhoI, 2132+850, L)
Sub into pBca9145-I716151 (Bg1II/XhoI, 2063+1221, S)
Sub into pBca9145-I716151 (Bg1II/XhoI, 2063+1221, S)
Product is I716161
Product is I716161
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Latest revision as of 21:23, 27 June 2007

Composite part of RBS (pBca1101-1106A) + hemA from R. capsulatus

Digest pBca1101-1106A 		        (BamH1/XhoI, 2132+850, L)
Sub into pBca9145-I716151		(Bg1II/XhoI, 2063+1221, S)
Product is I716161
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