From 2007.igem.org

Revision as of 21:54, 15 June 2007 (view source) Vaibhaviu (Talk | contribs) ← Older edit		Latest revision as of 20:53, 18 June 2007 (view source) Dtulga (Talk | contribs)
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	<pre>		<pre>
-	Composite part of RBS+hemB	+	Composite part of RBS (pBca1101-I716051-kan cassette) + hemB from Mg1655
	Digest pBca9145-I716153        (Bg1II/XhoI, 2063+990, S)		Digest pBca9145-I716153        (Bg1II/XhoI, 2063+990, S)
-	Sub into pBca9145-1716051 (BamH1/XhoI, 3119+850, L)	+	Sub into pBca1101-I716051 (BamH1/XhoI, 3119+850, L)
	Product is I716156</pre>		Product is I716156</pre>

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Latest revision as of 20:53, 18 June 2007

Composite part of RBS (pBca1101-I716051-kan cassette) + hemB from Mg1655

Digest pBca9145-I716153         (Bg1II/XhoI, 2063+990, S)
Sub into pBca1101-I716051	(BamH1/XhoI, 3119+850, L)
Product is I716156