http://2007.igem.org/wiki/index.php?title=ETHZ/primer_pcr_killspe_up&feed=atom&action=historyETHZ/primer pcr killspe up - Revision history2024-03-29T12:36:51ZRevision history for this page on the wikiMediaWiki 1.16.5http://2007.igem.org/wiki/index.php?title=ETHZ/primer_pcr_killspe_up&diff=34874&oldid=prevBrutsche at 21:33, 24 October 20072007-10-24T21:33:57Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 21:33, 24 October 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 2:</td>
<td colspan="2" class="diff-lineno">Line 2:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The sequence for the Primer to "kill" the SpeI site in the origin of replication is:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The sequence for the Primer to "kill" the SpeI site in the origin of replication is:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>CAA CTC AAA GGA AAA GGA CTA ATA ATT ATC ATT GAC TAG</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">5' </ins>CAA CTC AAA GGA AAA GGA CTA ATA ATT ATC ATT GAC TAG <ins class="diffchange diffchange-inline">3'</ins></div></td></tr>
</table>Brutschehttp://2007.igem.org/wiki/index.php?title=ETHZ/primer_pcr_killspe_up&diff=34866&oldid=prevBrutsche at 21:31, 24 October 20072007-10-24T21:31:42Z<p></p>
<p><b>New page</b></p><div>We made the site directed mutagenesis with help of PCR.<br />
The sequence for the Primer to "kill" the SpeI site in the origin of replication is:<br />
<br />
CAA CTC AAA GGA AAA GGA CTA ATA ATT ATC ATT GAC TAG</div>Brutsche