Edinburgh/DivisionPopper/Conclusions

From 2007.igem.org

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We have designed a device called Division PoPer that works as a function of bacterial division. The device reports when a bacterial division happens by generating a PoPS pulse on a single copy plasmid inserted in the bacteria.  
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We have designed a device called Division PoPer that works as a function of bacterial division. The device reports when a bacterial division happens by generating a PoPS pulse on a single copy plasmid inserted into the bacteria.  
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We designed and implemented it by following the paradigms of Synthetic Biology approach and we designed also a Proof of Concept device for proving the validity of the main assumptions and mechanisms.
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We designed and implemented it by following the paradigms of Synthetic Biology approach and we also designed a Proof of Concept device for proving the validity of the main assumptions and mechanisms.
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The most important mechanism is related to the dif sites, recombinatorial solutions that are employed universally by bacteria.
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The most important mechanism to test is related to the dif sites, recombinatorial solutions that are employed universally by bacteria.
We modelled both devices using classical and stochastic techniques and showed the quantitative and qualitative possibility of costructing such devices and composing it with a counter device.
We modelled both devices using classical and stochastic techniques and showed the quantitative and qualitative possibility of costructing such devices and composing it with a counter device.
In the wet lab we designed and constructed a total of thirteen biobricks that we registered and commented in the Registry.
In the wet lab we designed and constructed a total of thirteen biobricks that we registered and commented in the Registry.
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We look forward to meeting you all at the Jamboree.
We look forward to meeting you all at the Jamboree.

Revision as of 04:51, 27 October 2007

MENU : Introduction | Background | Applications | Design&Implementation | Modelling | Wet Lab | Synthetic Biology Approach | Conclusions


We have designed a device called Division PoPer that works as a function of bacterial division. The device reports when a bacterial division happens by generating a PoPS pulse on a single copy plasmid inserted into the bacteria. We designed and implemented it by following the paradigms of Synthetic Biology approach and we also designed a Proof of Concept device for proving the validity of the main assumptions and mechanisms. The most important mechanism to test is related to the dif sites, recombinatorial solutions that are employed universally by bacteria. We modelled both devices using classical and stochastic techniques and showed the quantitative and qualitative possibility of costructing such devices and composing it with a counter device. In the wet lab we designed and constructed a total of thirteen biobricks that we registered and commented in the Registry.

We look forward to meeting you all at the Jamboree.