Freiburg

From 2007.igem.org

(Difference between revisions)

Latest revision as of 14:55, 25 October 2007

Team Members:

Picture: The University of Freiburg iGEM Team in June 2007 (left to right: Natalia, Dinah, Corinna, Philipp, Katja, Maximilian, Moritz, Dario, Kristian)

Instructors:

Kristian M. Müller, Katja M.Arndt

Students:

Maximilian Mauler

Moritz Busacker

Corinna Gruber

Natalia Maier

Dario Hermida Aponte

Philipp Mappes

Graduate Student:

Dinah Mattay

Advisors:

Andreas Hiltbrunner, Michael Reth, Bodo Rak

Project Summary

Integrated Sensor-Executor Proteins and Molecular Switches
Our goal is to design integrated molecular sensing and executing devices based on modular protein engineering. These integrated devices can then easily be used for the construction of complex systems. We fuse sensing proteins, which provide nano-mechanical movements or dimerization upon an external signal, to executing proteins, which depend in their activity on the nano-mechanical change in the sensing part.
To elucidate the possibilities of such a system we used the calcium-ion sensor Calmodulin and the light sensor system PhyA-Fhy1. To test execution we used the split enzymes DHFR or beta-lactamase or the fluorescent proteins CFP and YFP, which can form a FRET pair. Sensors and executors were geneticlly fused and tesetd in E. coli for activity. So far we could demonstrate Ca2+ dependent growth of E. coli with the DHFR[1]-Calmodulin-DHFR[2] construct.
final iGEM report: Ca2+ sensor
final iGEM report: light sensor

Biobrick compatible strategy for fusion proteins
The present BioBrick prefix and suffix rules are not compatible with modular protein design. Thus, we propose an extension of the present standard for fusion proteins in which two restriction sites are added in frame adjacent to the coding sequence.
final iGEM report: Fusion parts

Support:

SYNBIOCOMM
Universität Freiburg
Wissenschaftliche Gesellschaft in Freiburg im Breisgau
GeneArt

Lab-Work:

lab notes1
lab notes2
lab notes3
plasmids
PCR primers
Lab Boxes

Protocols:

Mediums and Plates
DNA sequencing
Ligation
Plasmid spin column prep
Glycerol stocks
General Gene-Protein Information
Purification
Transformation
Dephosphorylation
Preparative Digestion
Analytic Digestion
Gel Electrophoresis
Polyacrylamide gel electrophoresis
Protein purification
In vivo test I
In vivo test II

Sandbox - wiki test:

sandbox

@@ Line 1: / Line 1: @@
 [[Image:BanneriGem.gif]]
 == Team Members: ==
-[[Image:FreiGEM Team 20070613 480.JPG]]
+<div style="float:right">
+{|
+|-
+| class="taxo-image" | [[Image:FreiGEM Team 20070613 480.JPG|thumb|398px|'''Picture:''' The University of Freiburg iGEM Team in June 2007 ''(left to right: Natalia, Dinah, Corinna, Philipp, Katja, Maximilian, Moritz, Dario, Kristian)'']]
+|}</div>
+<P><b>Instructors:</b></P>
+[https://2007.igem.org/User:Kristian Kristian M. Müller], Katja M.Arndt
+'''<P>Students:</P>'''
+[https://2007.igem.org/User:Maximilian Maximilian Mauler]
+[https://2007.igem.org/User:Moritz Moritz Busacker]
+Corinna Gruber
+Natalia Maier
+[https://2007.igem.org/User:Dario Dario Hermida Aponte]
+Philipp Mappes
+'''<P>Graduate Student:</P>'''
+Dinah Mattay
+'''<P>Advisors:</P>'''
+Andreas Hiltbrunner, Michael Reth, Bodo Rak
+== Project Summary ==
+<b>Integrated Sensor-Executor Proteins and Molecular Switches</b><br>
+Our goal is to design integrated molecular sensing and executing devices based on modular protein engineering. These integrated devices can then easily be used for the construction of complex systems. We fuse sensing proteins, which provide nano-mechanical movements or dimerization upon an external signal, to executing proteins, which depend in their activity on the nano-mechanical change in the sensing part.<br>
+To elucidate the possibilities of such a system we used the calcium-ion sensor Calmodulin and the light sensor system PhyA-Fhy1. To test execution we used the split enzymes DHFR or beta-lactamase or the fluorescent proteins CFP and YFP, which can form a FRET pair. Sensors and executors were geneticlly fused and tesetd in E. coli for activity. So far we could demonstrate Ca2+ dependent growth of E. coli with the DHFR[1]-Calmodulin-DHFR[2] construct.
+<br>
+[[Freiburg07/report_Ca_sensor| final iGEM report: Ca2+ sensor]]<BR>
+[[Freiburg07/report_light_sensor| final iGEM report: light sensor]]<BR>
+<b>Biobrick compatible strategy for fusion proteins</b><br>
+The present BioBrick prefix and suffix rules are not compatible with modular protein design. Thus, we propose an extension of the present standard for fusion proteins in which two restriction sites are added in frame adjacent to the coding sequence.<br>
+[[Freiburg07/report_fusion_parts| final iGEM report: Fusion parts]]<BR>
+== Support: ==
+[http://www.syntheticbiology.ethz.ch/synbiocomm/index SYNBIOCOMM]<br>
+[http://www.uni-freiburg.de/ Universität Freiburg]<br>
+[http://www.uni-freiburg.de/wiss-ges/ Wissenschaftliche Gesellschaft in Freiburg im Breisgau]<br>
+[http://www.geneart.com GeneArt]<br>
-Picture: The University of Freiburg iGEM Team in June 2007 (left to right: Natalia, Dinah, Corinna, Philipp, Katja, Maximilian, Moritz, Dario, Kristian)
+== Lab-Work: ==
+[[Freiburg07/labnotes1| lab notes1]]<BR>
+[[Freiburg07/labnotes2| lab notes2]]<BR>
+[[Freiburg07/labnotes| lab notes3]]<BR>
+[[Freiburg07/plasmids| plasmids]]<BR>
+[[Freiburg07/primers| PCR primers]]<BR>
+[[Freiburg07/boxes| Lab Boxes]]<BR>
-<P>Instructors:</P>
+== Protocols: ==
-[https://2007.igem.org/User:Kristian Kristian M. Müller]
-<P>Students:</P>
+[[Freiburg07/Mediums and Plates| Mediums and Plates]]<BR>
+[[Freiburg07/DNA_sequencing| DNA sequencing]]<BR>
+[[Freiburg07/Ligation| Ligation]]<BR>
+[[Freiburg07/Plasmidprep| Plasmid spin column prep]]<BR>
+[[Freiburg07/Glycerol_stock| Glycerol stocks]]<BR>
+[[Freiburg07/Gene_Protein_info| General Gene-Protein Information]]<BR>
+[[Freiburg07/Purification|Purification ]]<BR>
+[[Freiburg07/Transformation|Transformation ]]<BR>
+[[Freiburg07/Dephosphorylation|Dephosphorylation ]]<BR>
+[[Freiburg07/Preparative Digestion|Preparative Digestion ]]<BR>
+[[Freiburg07/Analytic Digestion|Analytic Digestion ]]<BR>
+[[Freiburg07/Gel Electrophoresis|Gel Electrophoresis ]]<BR>
+[[Freiburg07/Polyacrylamide gel electrophoresis|Polyacrylamide gel electrophoresis ]]<BR>
+[[Freiburg07/Protein purification|Protein purification ]]<BR>
+[[Freiburg07/In vivo test I|In vivo test I]]<BR>
+[[Freiburg07/In vivo test II|In vivo test II]]<BR>
+== Sandbox - wiki test: ==
+[[Freiburg07/sandbox| sandbox]]<BR>
+<!-- Hi allerseits -falls jemand das vor Freitag liest- hätte gerne Feedback:
+-wie detailliert soll das projekt hier beschrieben werden? andre teams halten damit eher hinterm berg...?
+-brauchen Bild von Molekülen, also falls jemand Lust auf Pymol hat...
+-soll ich versuchen, zwischen bild und liste nochmal das uni-logo mit link zur uni-page zu setzen?
+-wers will: richtet eure Seiten ein, sonst kann ich keine links machen...
+-@kristian: wie bezeichne ich die "synbiocomm" offiziell, und soll ich die verlinken (wenn ja, wohin?) konkrete Angaben zu erhaltenen Beträgen lass ich besser weg, oder? Bis dahin, Grüsse, Philipp
-<!-- Hallo Phillip & Corinna,
+KM: Hallo Philipp, ein link zur uni-page kann man auch separat in einem kurzen Text unter die Namen setzen, Höhe finanzieller Beiträge würde ich im Moment noch weg lassen, das Projekt würde ich aus einem der Anträge kopieren (der Text ist eine Idee). Die Plazierung des Bildes mit Unterschrift kann zusammengezogen werden ev als Tabelle.
-ich setzte das Bild und mein link schon mal ein, Ihr könnt das ja in eine brauchbare Form bringen. Neben der MediWiki Seite, bietet auch http://de.selfhtml.org/index.htm gute Hilfen.
+Grüße Kristian
-Grüße, Kristian -->
+-->

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