Freiburg

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Team Members:

Picture: The University of Freiburg iGEM Team in June 2007 (left to right: Natalia, Dinah, Corinna, Philipp, Katja, Maximilian, Moritz, Dario, Kristian)

Instructors:

Kristian M. Müller, Katja M.Arndt

Students:

Maximilian Mauler

Moritz Busacker

Corinna Gruber

Natalia Maier

Dario Hermida Aponte

Philipp Mappes

Graduate Student:

Dinah Mattay

Advisors:

Andreas Hiltbrunner, Michael Reth, Bodo Rak

Project Summary

Integrated Sensor-Executor Proteins and Molecular Switches
Our goal is to design integrated molecular sensing and executing devices based on modular protein engineering. These integrated devices can then easily be used for the construction of complex systems. We fuse sensing proteins, which provide nano-mechanical movements or dimerization upon an external signal, to executing proteins, which depend in their activity on the nano-mechanical change in the sensing part.
To elucidate the possibilities of such a system we used the calcium-ion sensor Calmodulin and the light sensor system PhyA-Fhy1. To test execution we used the split enzymes DHFR or beta-lactamase or the fluorescent proteins CFP and YFP, which can form a FRET pair. Sensors and executors were geneticlly fused and tesetd in E. coli for activity. So far we could demonstrate Ca2+ dependent growth of E. coli with the DHFR[1]-Calmodulin-DHFR[2] construct.


Biobrick compatible strategy for fusion proteins

Support:

SYNBIOCOMM

Universität Freiburg, Wissenschaftliche Geselschaft in Freiburg im Breisgau

GeneArt

Lab-Work:

lab notes
plasmids
PCR primers
Lab Boxes

Protocols:

DNA sequencing
Plasmid spin column prep
Glycerol stocks
General Gene-Protein Information

Sandbox - wiki test: