Freiburg07/Dephosphorylation

From 2007.igem.org

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Add one more 1 µl SAP.<BR>
Add one more 1 µl SAP.<BR>
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Incubate for 20 minutes at 70°C (heat shock)<br>
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Incubate for 20 minutes at 70°C (heat shock).<br>
[https://2007.igem.org/Freiburg BACK]
[https://2007.igem.org/Freiburg BACK]

Revision as of 15:19, 24 October 2007

Dephosphorylation

total volume: 50 µl
43 µl plasmid
1 µl SAP (shrink alkaline phosphatase)
5 µl SAP buffer (1:10)

Incubate for 30 minutes at 37°C.

Add one more 1 µl SAP.

Incubate for 20 minutes at 70°C (heat shock).

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