Freiburg07/Dephosphorylation

From 2007.igem.org

Revision as of 15:19, 24 October 2007 by Luckytaschka (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Dephosphorylation

total volume: 50 µl
43 µl plasmid
1 µl SAP (shrink alkaline phosphatase)
5 µl SAP buffer (1:10)

Incubate for 30 minutes at 37°C.

Add one more 1 µl SAP.

Incubate for 20 minutes at 70°C (heat shock)

BACK