Freiburg07/Transformation

From 2007.igem.org

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'''Transformation of frozen competent cells'''
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'''Transformation of frozen chem. competent cells'''
1. Thaw frozen cells.<BR>
1. Thaw frozen cells.<BR>
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2. Add 10 µl ligation and shake gent<BR>
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2. Add 10 µl ligation and shake gently.<BR>
3. Incubate on ice for 5-10 minutes.<br>
3. Incubate on ice for 5-10 minutes.<br>
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4. Heat shock cells for 10 minutes at 37 °C.
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4. Heat shock cells for 10-20 minutes at 37 °C.<BR>
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5. Bring up in 1 mL LB and shake gently for 1 hour at 37 °C.
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5. Add 900 µl DYT-Medium and shake gently for 60-70 minutes at 37 °C.<br>
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[https://2007.igem.org/Freiburg BACK]

Latest revision as of 15:00, 24 October 2007

Transformation of frozen chem. competent cells

1. Thaw frozen cells.

2. Add 10 µl ligation and shake gently.

3. Incubate on ice for 5-10 minutes.

4. Heat shock cells for 10-20 minutes at 37 °C.

5. Add 900 µl DYT-Medium and shake gently for 60-70 minutes at 37 °C.

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