Freiburg07/Transformation

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Transformation of frozen competent cells

1. Thaw frozen cells.

2. Add 10 µl ligation and shake gent

3. Incubate on ice for 5-10 minutes.

4. Heat shock cells for 10 minutes at 37 °C.

5. Bring up in 1 mL LB and shake gently for 1 hour at 37 °C.