Freiburg07/plasmids

From 2007.igem.org

(Difference between revisions)
(Origin plasmids)
(Obtained plasmids)
 
(30 intermediate revisions not shown)
Line 1: Line 1:
-
== Origin plasmids ==
+
== Plasmids obtained from others==
-
Sequence of Calmodulin: [[Freiburg07/Cam_yc6E_2|Cam_yc6E_2 ]]<BR>
+
 
 +
'''Ca2+ sensor: ß-lactamase'''<br>
Plasmid containing the lactamase part 1 fragment: [[Freiburg07/pAR310d-blac1-winzipA2|pAR310d-blac1-winzipA2 ]]<BR>
Plasmid containing the lactamase part 1 fragment: [[Freiburg07/pAR310d-blac1-winzipA2|pAR310d-blac1-winzipA2 ]]<BR>
-
Plasmid containing the lactamase part 1 fragment with periplasmatic signal sequence: [[Freiburg07/| ]]<BR>
+
Plasmid containing the lactamase part 1 fragment with periplasmatic signal sequence: [[Freiburg07/pAR310dp-blac1-winzipA2| pAR310dp-blac1-winzipA2]]<BR>
Plasmid containing the lactamase part 2 fragment: [[Freiburg07/pAR300d-winzipB1-blac2| pAR300d-winzipB1-blac2]]<BR>
Plasmid containing the lactamase part 2 fragment: [[Freiburg07/pAR300d-winzipB1-blac2| pAR300d-winzipB1-blac2]]<BR>
Plasmid containing the lactamase part 2 fragment with periplasmatic signal sequence: [[Freiburg07/pAR300dp-winzipB1-blac2| pAR300dp-winzipB1-blac2]]<BR>
Plasmid containing the lactamase part 2 fragment with periplasmatic signal sequence: [[Freiburg07/pAR300dp-winzipB1-blac2| pAR300dp-winzipB1-blac2]]<BR>
 +
 +
 +
'''Ca2+ sensor: DHFR'''<br>
Plasmid containing the dhfr1 fragment: [[Freiburg07/pAR200d-GAS41reg1-dhfr1|pAR200d-GAS41reg1-dhfr1 ]]<BR>
Plasmid containing the dhfr1 fragment: [[Freiburg07/pAR200d-GAS41reg1-dhfr1|pAR200d-GAS41reg1-dhfr1 ]]<BR>
Plasmid containing the dhfr2 fragment: [[Freiburg07/pAR300d-AF10winDelV41D-dhfr2A|pAR300d-AF10winDelV41D-dhfr2A ]]<BR>
Plasmid containing the dhfr2 fragment: [[Freiburg07/pAR300d-AF10winDelV41D-dhfr2A|pAR300d-AF10winDelV41D-dhfr2A ]]<BR>
 +
 +
 +
'''Light sensor: Phytochrom A with CFP and YFP'''<br>
Plasmid containing PhyA1: [[Freiburg07/D153ah-phyA_1-406| D153ah-phyA_1-406 ]]<BR>
Plasmid containing PhyA1: [[Freiburg07/D153ah-phyA_1-406| D153ah-phyA_1-406 ]]<BR>
Line 25: Line 32:
Plasmid containing YFP: [[Freiburg07/ pAR200-YFP-Dumy|  pAR200-YFP-Dumy ]]<BR>
Plasmid containing YFP: [[Freiburg07/ pAR200-YFP-Dumy|  pAR200-YFP-Dumy ]]<BR>
-
== New plasmids ==
+
 
 +
'''Sequence of Calmodulin''': [[Freiburg07/Cam_yc6E_2|Cam_yc6E_2 ]]<BR>
 +
<br>
 +
 
 +
== New plasmids (engineered by iGEM-Team-Members)==
 +
 
 +
 
 +
Plasmid wih both lactamase fragments:[[Freiburg07/ pFR320d-blac1-winzipB1-blac2| pFR320d-blac1-winzipB1-blac2 ]]<BR>
 +
Plasmid wih both lactamase fragments and periplasmatic signal sequence:[[Freiburg07/pFR320p_b1_WinzipB1_b2| pFR320p_b1_WinzipB1_b2 ]]<BR>
 +
 
 +
 
 +
'''Ca2+ sensor: ß-lactamase''' <br>
 +
 
 +
 
 +
Plasmids with calmodulin between the lactamase fragments:<BR>
 +
 
 +
2 amino acids for linker: [[Freiburg07/pFR320p_b1_2calmo2_b2| pFR320p_b1_2calmo2_b2]]<BR>
 +
4 amino acids for linker: [[Freiburg07/pFR320p_b1_4calmo4_b2| pFR320p_b1_4calmo4_b2]]<BR>
 +
6 amino acids for linker: [[Freiburg07/pFR320p_b1_6calmo6_b2| pFR320p_b1_6calmo6_b2]]<BR>
 +
 
 +
 
 +
'''Ca2+ sensor: DHFR'''<br>
 +
 
 +
Plasmids with calmodulin between the dhfr fragments:<BR>
 +
 
 +
2 amino acids for linker: [[Freiburg07/dFR320d-dhfr1-2calmo2-dhfr2A|dFR320d-dhfr1-2calmo2-dhfr2A ]]<BR>
 +
4 amino acids for linker: [[Freiburg07/dFR320d-dhfr1-4calmo4-dhfr2A|dFR320d-dhfr1-4calmo4-dhfr2A ]]<BR>
 +
6 amino acids for linker: [[Freiburg07/dFR320d-dhfr1-6calmo6-dhfr2A|dFR320d-dhfr1-6calmo6-dhfr2A ]]<BR>
 +
 
 +
 
 +
'''Light sensor: Phytochrom A with CFP and YFP'''<br>
 +
 
[[Freiburg07/pAR200-Fhy1Big-YFP| pAR200-Fhy1Big-YFP]]<BR>
[[Freiburg07/pAR200-Fhy1Big-YFP| pAR200-Fhy1Big-YFP]]<BR>
Line 32: Line 70:
[[Freiburg07/pAR200-YFP-Fhy1Big|pAR200-YFP-Fhy1Big ]]<BR>
[[Freiburg07/pAR200-YFP-Fhy1Big|pAR200-YFP-Fhy1Big ]]<BR>
[[Freiburg07/pAR200-YFP-Fhy1Small|pAR200-YFP-Fhy1Small ]]<BR>
[[Freiburg07/pAR200-YFP-Fhy1Small|pAR200-YFP-Fhy1Small ]]<BR>
 +
<br>
-
Plasmid wih both lactamase fragments:[[Freiburg07/pAR200-YFP-Fhy1Small|pAR200-YFP-Fhy1Small ]]<BR>
+
[https://2007.igem.org/Freiburg BACK]
-
Plasmid wih both lactamase fragments and periplamatic signal sequence:[[Freiburg07/pAR200-YFP-Fhy1Small|pAR200-YFP-Fhy1Small ]]<BR>
+

Latest revision as of 15:51, 24 October 2007

Plasmids obtained from others

Ca2+ sensor: ß-lactamase

Plasmid containing the lactamase part 1 fragment: pAR310d-blac1-winzipA2

Plasmid containing the lactamase part 1 fragment with periplasmatic signal sequence: pAR310dp-blac1-winzipA2

Plasmid containing the lactamase part 2 fragment: pAR300d-winzipB1-blac2

Plasmid containing the lactamase part 2 fragment with periplasmatic signal sequence: pAR300dp-winzipB1-blac2


Ca2+ sensor: DHFR

Plasmid containing the dhfr1 fragment: pAR200d-GAS41reg1-dhfr1

Plasmid containing the dhfr2 fragment: pAR300d-AF10winDelV41D-dhfr2A


Light sensor: Phytochrom A with CFP and YFP

Plasmid containing PhyA1: D153ah-phyA_1-406

Plasmid containing FHY1: pGADT7ah-FHY1

Plasmid containing CFP: pAR200-cJunCFP

Plasmid containing YFP: pAR200-cFos-YFP

Plasmid containing YFP: pAR200-YFP-Dumy


Sequence of Calmodulin: Cam_yc6E_2

New plasmids (engineered by iGEM-Team-Members)

Plasmid wih both lactamase fragments: pFR320d-blac1-winzipB1-blac2
Plasmid wih both lactamase fragments and periplasmatic signal sequence: pFR320p_b1_WinzipB1_b2


Ca2+ sensor: ß-lactamase


Plasmids with calmodulin between the lactamase fragments:

2 amino acids for linker: pFR320p_b1_2calmo2_b2
4 amino acids for linker: pFR320p_b1_4calmo4_b2
6 amino acids for linker: pFR320p_b1_6calmo6_b2


Ca2+ sensor: DHFR

Plasmids with calmodulin between the dhfr fragments:

2 amino acids for linker: dFR320d-dhfr1-2calmo2-dhfr2A
4 amino acids for linker: dFR320d-dhfr1-4calmo4-dhfr2A
6 amino acids for linker: dFR320d-dhfr1-6calmo6-dhfr2A


Light sensor: Phytochrom A with CFP and YFP


pAR200-Fhy1Big-YFP
pAR200-Fhy1Small-YFP
pAR200-PhyA-His-CFP
pAR200-YFP-Fhy1Big
pAR200-YFP-Fhy1Small

BACK