Glasgow/Wetlab

From 2007.igem.org

(Difference between revisions)
(03/07)
(04/07)
Line 13: Line 13:
#Reporter constructs and mini-Tn5 stocks looked out.
#Reporter constructs and mini-Tn5 stocks looked out.
#Streaked the following:
#Streaked the following:
-
##P. putida PAW 340 pJAK14 Carb. plate
+
*P. putida PAW 340 pJAK14 Carb. plate
##Tn5 lux AB Carb. plate
##Tn5 lux AB Carb. plate
##Mini-Tn5 lux AB Carb. plate
##Mini-Tn5 lux AB Carb. plate

Revision as of 16:29, 19 July 2007

PROTOCOLSREFERENCES

Week 1

03/07

  1. Maija and Christine prepared LB broth and LB agar with Protocol 1. Made 4 batches of plates, 2 with kanamycin and 2 with carbenicillin.

04/07

  1. All wetlab researched BioBricks.
  2. Reporter constructs and mini-Tn5 stocks looked out.
  3. Streaked the following:
  • P. putida PAW 340 pJAK14 Carb. plate
    1. Tn5 lux AB Carb. plate
    2. Mini-Tn5 lux AB Carb. plate
    3. P. Fluorescens NCIMB 9815 Carb. plate
    4. P. putida KT2440 LB
    5. JM109 pBluescript 5k+ Carb. plate
    6. Mini-Tn5 Tc Carb. Plate
    7. pQF52 Carb. plate
    8. P. Fluorescens 9815 LB
    9. E. coli pJAK14 Km plate
    10. Il DntR in pOF52 Carb. plate
    11. pUCINR in Ω strain C Carb. plate
    12. pGLTUR Carb. plate
    13. Mini-Tn5 Kan Carb. plate
    14. Mini-Tn5 Sm/Sp Carb. plate
    15. Mini-Tn5 1cc2 Carb. plate
    16. E. coli Sa1 LB
    17. DmpR #24 Carb. plate
    18. Mini-Tn5 lac 32 in E. coli 517 Carb. plate
    19. Mini-Tn5 Tc Carb. Plate
    20. Mini-Tn5 Cm Carb. plate
    21. DmpR WT Carb. plate
    22. E.coli sm 10 pESD15 Tn5 GFP Carb. plate
    23. pUJ8 Carb. Plate