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	#Reporter constructs and mini-Tn5 stocks looked out.		#Reporter constructs and mini-Tn5 stocks looked out.
	#Streaked the following:		#Streaked the following:
-	*P. putida PAW 340 pJAK14 Carb. plate	+	**P. putida PAW 340 pJAK14 Carb. plate
	##Tn5 lux AB Carb. plate		##Tn5 lux AB Carb. plate
	##Mini-Tn5 lux AB Carb. plate		##Mini-Tn5 lux AB Carb. plate

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Revision as of 16:29, 19 July 2007

PROTOCOLSREFERENCES

Week 1

03/07

1. Maija and Christine prepared LB broth and LB agar with Protocol 1. Made 4 batches of plates, 2 with kanamycin and 2 with carbenicillin.

04/07

1. All wetlab researched BioBricks.
2. Reporter constructs and mini-Tn5 stocks looked out.
3. Streaked the following:

• • P. putida PAW 340 pJAK14 Carb. plate

1. 1. Tn5 lux AB Carb. plate
   2. Mini-Tn5 lux AB Carb. plate
   3. P. Fluorescens NCIMB 9815 Carb. plate
   4. P. putida KT2440 LB
   5. JM109 pBluescript 5k+ Carb. plate
   6. Mini-Tn5 Tc Carb. Plate
   7. pQF52 Carb. plate
   8. P. Fluorescens 9815 LB
   9. E. coli pJAK14 Km plate
   10. Il DntR in pOF52 Carb. plate
   11. pUCINR in Ω strain C Carb. plate
   12. pGLTUR Carb. plate
   13. Mini-Tn5 Kan Carb. plate
   14. Mini-Tn5 Sm/Sp Carb. plate
   15. Mini-Tn5 1cc2 Carb. plate
   16. E. coli Sa1 LB
   17. DmpR #24 Carb. plate
   18. Mini-Tn5 lac 32 in E. coli 517 Carb. plate
   19. Mini-Tn5 Tc Carb. Plate
   20. Mini-Tn5 Cm Carb. plate
   21. DmpR WT Carb. plate
   22. E.coli sm 10 pESD15 Tn5 GFP Carb. plate
   23. pUJ8 Carb. Plate