Glasgow/Wetlab/DryWeek1

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Contents

Week 1

02/07

After a brief re-introduction to the Laboratory and our project proposal, we outlined a [Glasgow/6-PHASE 6-PHASE] approach to guide our practice over the summer.

From here the Modellers began working on basic Matlab modelling tutorials, designed by Xu Gu, to allow all modellers to reach a satisfactory ability. By the end of the day we had completed a number of [http://en.wikipedia.org/wiki/Law_of_mass_action Mass-action] programs using the ode45 funtion and grasped the translation from basic notaion into Substrate, Enzyme and S/E-complex notation.

03/07

We developed our modelling techniques by programming responses to basic [http://en.wikipedia.org/wiki/Metabolic_pathway metabolic] and [http://en.wikipedia.org/wiki/Cell_signaling signalling] pathways. We then learnt more precise techniques of modelling, e.g. accuracy and tolerace variance and noting parameters. We then covered Loop and Switch functions.

04/07

We were introduced to the 'Nested Functions' to allow for simpler programming, and the basic ideas behind Sensitivity of output due to a range of possible values of varying constants.

In the afternoon, all modellers were shown some Wetlab techniques for the sake of a more thorough understanding of the processes involved.

Our experiment was to extract plasmids from a number of different bacterial cultures.

05/07

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Tutorials *References* *Resources* *Orders* *Biobricks*
Used
*Gels*